How Pillar Plate Works for 3D Cell Culture and Analysis
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Simple and robust cell encapsulation in hydrogels on pillars: Sidewalls and slits ensure reproducible and robust loading of cells and spheroids in hydrogels on pillars, preventing cell spot detachment and supporting long-term 3D cell and organoid culture.
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Minimum assay volume: Each pillar accommodates an optimum of 5 µL of hydrogel without spillage.
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Uniform nutrients and oxygen diffusion: The small volume of the pillar plate facilitates uniform diffusion of nutrients and oxygen, enhancing cell growth, differentiation, maturation, and function.
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High flexibility in 3D cell and organoid culture and analysis: The pillar plates can be sandwiched with the perfusion plates as well as standard 384-well plates interchangeably, enabling flexibility in 3D cell and organoid culture and analysis.
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On-chip cell testing, staining, and imaging: The pillar plate with 3D-cultured cells can be tested with compounds, stained with fluorescent dyes and antibodies, and imaged with standard fluorescence microscopes at 4x and 10x magnification for high-content imaging (HCI) and immunofluorescence assays.
Key Features and Benefits
- Rapid and robust cell/spheroid loading and encapsulation: Single cells and spheroids in hydrogels can be loaded on the pillar plate by manual dispensing, pillar plate stamping, spheroid transferring, and microarray 3D bioprinting.
- High-throughput microarray 3D bioprinting: Enables layer-by-layer dispensing of cells and spheroids in hydrogels on the pillar plate for the scale-up production of organoids.
- Flexible 3D cell culture approaches: 3D cells and organoids can be cultured statically or dynamically by sandwiching pillar plates with deep well plates and perfusion plates.
- Advanced surface functionalization: Hydrophilic coatings on the pillar plate prevent air bubble entrapment and cell spot detachment, ensuring long-term 3D cell cultures in hydrogels.
- On-chip cryopreservation of human organoids: 3D cells and organoids cultured on the pillar plate can be cryopreserved directly for long-term storage and on-demand delivery of assay-ready pillar plates.
- Scalable throughput options: Available in 36-, 144-, and 384-pillar formats to support varying research and screening needs.
Modular Design for Versatile Applications
Supports both static and dynamic cultures under various test conditions.
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